By using the luciferase reporter, we screened a few small molecule compounds of anti-endonuclease that inhibited the nicking activity by parvovirus B19 (B19V) NS1, as well as FDA-approved drugs targeting the RdRP of influenza virus. In this study, we reconstituted a replicon reporter system, composed of plasmids expressing the RNA-dependent RNA polymerase (RdRP) complex (PA, PB1, and PB2), nucleocapsid (NP) protein, and a reporter gene flanked by the 3′ and 5′ untranslated region (UTR) of the envelope glycoprotein (GP) genome segment. Currently, there are no treatments or vaccines available for BRBV or thogotovirus infection caused diseases. It belongs to genus Thogotovirus of the Orthomyxoviridae family.
BRBV is a tick-borne virus that is widely carried by lone star ticks.
Since then, several deaths have been reported to be caused by BRBV infection in the Midwest and Southern United States. Louis, MO, United Statesīourbon virus (BRBV) was first isolated from a patient hospitalized at the University of Kansas Hospital in 2014.
Siyuan Hao 1, Kang Ning 1, Xiaomei Wang 1, Jianke Wang 1, Fang Cheng 1, Safder S.